mitobright deep red mt12  (Dojindo Labs)

Journal: Advanced Science

Article Title: Nature‐Inspired Surface Modification Strategy Reverses the Autophagic Flux Impairment of Mitochondrial Transplantation for Attenuating Ischemic Strokes

doi: 10.1002/advs.202518969

Figure Lengend Snippet: Construction and characterization of SR‐coated mitochondria (MSR) (a) Schematic illustration of the construction of MSR. (b) Confocal laser scanning microscope (CLSM) images of MitoTracker‐stained mitochondria (red) and C6‐labeled SR (green). Scale bars: 25 µm. (c) Representative snapshots of molecular dynamics simulation of the interactions between SR and the mitochondrial outer membrane. (d) Snapshot of interactions between SR and the mitochondrial outer membrane at a simulation time of 100 ns. The enlarged view highlighted the hydrophobic interactions (blue box) and hydrogen‐bonding interactions (red box). (e) Time‐dependent changes in RMSD for SR during the interactions with the mitochondrial outer membrane. (f) Time‐dependent changes of the electrostatic effect and the Van der Waals effect. (g) TEM images of unmodified mitochondria and MSR. Scale bars: 200 nm. (h) Size distribution of unmodified mitochondria and MSR (t‐test; n = 35 per group). (i) Quantitative analysis of unmodified mitochondria and MSR for mitochondrial membrane potential after staining with JC‐1 (t‐test; n = 3 per group). Data are presented as mean ± s.d., and n represents biological replicates. * p <0.05; ** p <0.01; *** p <0.001; **** p <0.0001 are considered as statistically significant.

Article Snippet: Moreover, exogenous mitochondria were incubated with MitoTracker (MitoBright LT Deep Red, DOJINDO, MT12, Ex: 640 nm/Em: 650–700 nm) with slow shaking at 37 °C for 30 mins.

Techniques: Laser-Scanning Microscopy, Staining, Labeling, Membrane

Journal: Advanced Science

Article Title: Nature‐Inspired Surface Modification Strategy Reverses the Autophagic Flux Impairment of Mitochondrial Transplantation for Attenuating Ischemic Strokes

doi: 10.1002/advs.202518969

Figure Lengend Snippet: MLSR enabled brain‐targeted mitochondrial transplantation. (a) Schematic illustration of the in vitro BBB model for evaluating BBB penetration of MSR and MLSR. (b) CLSM images showed the enhanced transport efficiency facilitated by Lf modification in the in vitro BBB model. Scale bar: 50 µm. (c) Quantification of fluorescence intensity from the lower chamber of the in vitro BBB model. (n = 3 per group, t‐test). (d) In vivo fluorescence imaging of Ce6‐labeled MSR and MLSR in the brain region post‐injection. (e) Quantification of the fluorescence intensity in the brain region from the MSR and MLSR group (n = 3 per group). (f) Quantification of fluorescence intensity from ex vivo brains at 3 h post‐injection. (t‐test; n = 3 per group). (g) Quantification of fluorescence intensity in ex vivo major organs at 3 h post‐injection (t‐test; n = 3 per group). (h) CLSM images and the fluorescence quantification showed a greater accumulation of Mitotracker‐labeled MLSR in the ischemic penumbra after intravenous injection (right side). The same imaging parameters, including identical exposure times and Look‐Up Table (LUT) were employed for different groups to ensure fair comparison (t‐test; n = 3 per group). Scale bar: 2 mm. Data are presented as mean ± s.d., and n represents biological replicates. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 are considered as statistically significant.

Article Snippet: Moreover, exogenous mitochondria were incubated with MitoTracker (MitoBright LT Deep Red, DOJINDO, MT12, Ex: 640 nm/Em: 650–700 nm) with slow shaking at 37 °C for 30 mins.

Techniques: Transplantation Assay, In Vitro, Modification, Fluorescence, In Vivo, Imaging, Labeling, Injection, Ex Vivo, Comparison